An Ytterbium-doped mode-locked fiber laser using the polarization sensitive isolator and polarization controllers (PC) is reported. In the normal dispersion, the fiber laser can oscillate at the multi-pulse states by adjusting the position of the PC.On the condition that the polarization state isn't changed, the outputs of Ytterbium-doped mode-locked fiber laser change from single pulse to multi-pulse by increasing the pump power. The fiber laser can achieve different multi-pulse output by adjusting the PC1 and PC2 carefully.